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Figure 8.1. Cell culture for ligament tissue engineering. A. Step 1 Cell expansion: Human BMSCs were plated on tissue culture plastic at a density of 5000/cm2 in DMEM-10% FCS containing 1 ng/ml bFGF (functions as the mitogen to enhance BMSC proliferation and as differentiation inhibitor) and culture-expanded for 6 days (Chen et al, 2003). B. Step 2 Cell seeding: Before con uence, BMSCs were trypsinized, centrifuged, and resuspended at a concentration of 2 million/ml in DMEM-10% FCS. BMSCs were seeded onto RGD-modi ed silk matrices in a custom-designed seeding vessel (Chen et al, 2003) to increase cell-matrix contact. C. As early as 5 min after seeding, BMSCs attached to RGD-modi ed silk matrices and started to spread (Chen et al, 2003). D. Step 3 Cell cultivation on silk matrices: Two hours after seeding, matrices were transferred from seeding vessel into tissue culture asks, followed by a 2-week cultivation in DMEM-10% FCS at 37 C/5% CO2 . E. After the 2-week static culture, BMSCs reached con uence and fully covered the silk matrix. Cell/ECM was not aligned (Chen et al, 2003). F. Step 4 Bioreactor and mechanical stimulation: Cell-seeded silk matrices were then transferred into bioreactor and cultured under dynamic conditions with direct cyclic mechanical stimulation for 6 days (Altman et al, J Biomech Eng. 2002 Dec;124(6):742 9). Culture medium was re ushed for 1 h/day. G. The mechanically stimulated matrices showed highly organized cell/ECM alignment (Chen et al, 2003).

Click the tab of the sheet you want to move, making sure to hold down the mouse button. Drag the tab to the position you desire by using the arrows for reference (see Figure 9-4). When the arrow is pointing to your desired location, release the mouse button.

Before we examine each of these techniques in more detail, let s rst understand the nature of the challenges. 4.3.1 Challenges Facing Reliable Multicast

cw(v)

Table B.5 Minimal polynomials of all the elements of the Galois eld GF(24 ) generated by pi (X ) = 1 + X + X 4 Conjugate roots 0 1 , 2 , 4 , 8 3 , 6 , 9 , 12 5 , 10 7 , 11 , 13 , 14 Minimal polynomials X 1+ X 1 + X + X4 1 + X + X2 + X3 + X4 1 + X + X2 1 + X3 + X4

4.2. Genetically Engineered Selectable Markers A technique that is currently under development to enrich particular cell types is to engineer a cell s gene expression so that the desired progeny is enriched [Friedrich and Soriano, 1991; Moritoh et al., 2003; Soria et al., 2000]. This process of enrichment can be induced either through the activation of suicide genes on the expression of particular genes or the expression of genes that maintain the cells. For example, neomycin resistance (neo, aminoglycoside phosphotransferase) can be engineered into ES cells in a construct with a lineage-speci c promoter. The expression of neomycin resistance can then be regulated by the promoters that are

If you receive a warning message about the le being too large, you need to use a oppy disk or CD with more free space or a much larger disk such as an external hard drive.

To create or edit a solid color with the Color Mixer:

Select one of the visualization categories, such as Ambience, Particle, or Musical Colors. Click the name of a speci c visualization (see Figure 12-6). It appears in the main window of Windows Media Player and pulsates to the beat of your song. Try each of the visualizations in the numerous categories to nd one that pleases you.

Documentation for the FileSystemObject object is available online, at:

Once again, I ve provided the tcp dump output so that you can see where the data is being hidden. Because I am using TCP and every TCP packet is embedded in an IP packet and the standard size of the IP packet is 20 bytes, you have to count 20 bytes to get to the beginning of the TCP header. Then, starting at 0, you count to the fourth and fifth byte, which contain the sequence number and our data, which I ve highlighted in the tcp dump.

Table 2-4

If you have previous code that expects to directly control the motors, you can solve the equations for V and r and use them in the DRIVE command.